Composite

Part:BBa_K5062008:Design

Designed by: Christian Nelson Tejedor Vicera   Group: iGEM24_HKU-HongKong   (2024-09-27)


5th Generation Chimeric Antigen Receptor (CAR) targeting GPC-3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3334
    Illegal SpeI site found at 1651
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3334
    Illegal SpeI site found at 1651
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3334
    Illegal BamHI site found at 54
    Illegal BamHI site found at 351
    Illegal XhoI site found at 471
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3334
    Illegal SpeI site found at 1651
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3334
    Illegal SpeI site found at 1651
    Illegal AgeI site found at 60
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1406
    Illegal BsaI.rc site found at 911
    Illegal BsaI.rc site found at 2618
    Illegal SapI.rc site found at 2823


Design Notes

Some are from genomic sequences, some are found from papers.


Source

The entire sequence is a large open reading frame that splits into several proteins upon translation. It is specifically designed for use in human cells

References